- Educate providers and the public about transmission and prevention of
Enterohemorrhagic Escherichia coli (EHEC).
- Educate providers and laboratories to report EHEC infections from
any site to the local health department in the patient's county of
residence within 24 hours of diagnosis.
- Educate laboratories to submit all EHEC isolates to the Office of
Laboratory Services (OLS) for serotyping and Pulsed Field Gel
Electrophoresis (PFGE).
- Educate laboratories about appropriate testing and referral of EHEC,
to include one of the following options:
- Screen stools with sorbitol MacConkey (SAMC) agar and an O157 latex
agglutination test. Sorbitol negative, O157 latex agglutination positive
isolates should be presumptively identified as E. coli O157:H7 and
referred to OLS for confirmation and PFGE; or
- By special arrangement, OLS may accept broth/slant from laboratories
that run an EIA test and identify Shiga toxin producing E. coli. Contact
the Office of Laboratory Services at (304) 558-3530 to make arrangements.
- Conduct an appropriate investigation as follows:
- For sporadic cases of EHEC: Complete the Supplemental Enteric Disease
Case Report Follow-up Form and attach it to the yellow card. Laboratory
results should also be attached. Use of the Supplemental Enteric Disease
Case Report Follow-up Form will prompt the local health department to
complete an appropriate investigation, including but not limited to: 1) a
two- to eight-day food history; 2) identification of high-risk persons or
symptomatic individuals for further investigation; and 3) identification
of specific behaviors that may be associated with EHEC.
- For small outbreaks (three or more epi-linked cases): Do not wait for
serotyping to begin the epi investigation as in 5a above; initiate active
surveillance. Consult the Infectious Disease Epidemiology Program (IDEP).
Take special care to assure that isolates are rapidly sent to the OLS for
serotyping and PFGE.
- For large outbreaks (five or more epi-linked cases): Consult IDEP
immediately. Take special care to assure that isolates are rapidly sent to
OLS for serotyping and PFGE.
- Identify other cases including probable cases (symptomatic persons
who are epidemiologically linked to a culture-confirmed case), and
investigate completely as in 5a above. Take care to collect stool samples
and forward them to OLS.
- Identify persons with E. coli O157:H7 who are employed in high-risk
professions:
- Exclude symptomatic individuals who are involved in food handling, and
from direct care of infants, elderly, immunocompromised, institutionalized
patients, and children enrolled in day care.
- Exclude asymptomatic individuals with questionable hygiene.
- Excluded individuals should only be returned to work after two
consecutive negative stool cultures are collected at least 24 hours apart
and 48 hours after the last dose of antibiotics.
- Identify household contacts of culture-confirmed cases. Culture the
stools of any household or close contacts who are involved in food
handling, direct patient care, or care of young children or the elderly in
institutional settings.
To reduce the risk of secondary or additional cases by:
- Early identification and appropriate exclusion of infected persons
from high risk situations (day care, food handling, health care).
- Rapid and complete investigation of outbreaks so that any common
source can be identified and removed.
To reduce the risk of infection from E. coli by:
- Education of the general public about hand washing as a primary
means of preventing person-to-person transmission of E. coli.
- Education of the general public about proper food handling,
including thorough cooking of ground meat and washing of fruits and
vegetables prior to consumption, and avoidance of
cross-contamination.
- Education of the general public to avoid unsafe foods such as
unpasteurized milk, cheese, juice, cider, and untreated water.
- To determine the incidence of E. coli in West Virginia.
- To identify demographic characteristics of persons with E. coli.
- To identify behavioral risk factors associated with E. coli.
- To facilitate outbreak identification and investigation by running
PFGE at OLS on all isolates.
Each year there are approximately 73,000 cases and 61 deaths due to
EHEC in the United States. Infected individuals usually have bloody
diarrhea, and some may experience kidney failure due to hemolytic uremic
syndrome (HUS). The most common cause of transmission of E. coli
O157:H7 is from the consumption of undercooked contaminated ground beef.
Person to person contact in families and day care centers can also
transmit the bacteria. One can also obtain the bacteria after drinking raw
milk and swimming in or drinking sewage-contaminated water.
EHEC has been responsible for large and dramatic outbreaks, including
the following:
- An EHEC outbreak occurred between 1992 and 1993 which resulted in
500 laboratory confirmed infections and four deaths occurring in four
states: Washington, Idaho, California, and Nevada. This outbreak was
due to the consumption of undercooked hamburgers from one restaurant
chain.
- In 1998, at least 26 children became infected with EHEC resulting in
one death after playing at an Atlanta water park that became
contaminated due to a fecal accident.
- Between June and July of 1997, simultaneous outbreaks of EHEC
occurred in Michigan and Virginia, involving 108 individuals who had
eaten alfalfa sprouts.
- An EHEC outbreak in July of 1997 resulted in 20 individuals becoming
ill in Colorado. This led to a recall of 25 million pounds of
suspected ground beef, which was the largest recall in U.S. history.
- In 1994, 18 summer camp attendees in Virginia became ill with EHEC
after the consumption of undercooked ground beef.
- The largest outbreak of EHEC occurred in 1996 in Japan affecting
6,309 school children and 92 school staff members from 62 elementary
schools. An additional 160 cases were reported among family members of
school children. There was a total of 9,578 cases of EHEC, of which
there were 101 cases of HUS which resulted in 11 deaths.
E. coli O157:H7 usually causes severe bloody diarrhea and
abdominal cramps; sometimes the infection causes non-bloody diarrhea or no
symptoms. There is usually little or no fever, and the illness resolves in
five to 10 days.
For children under five years of age and the elderly, the infection can
also cause a complication called hemolytic uremic syndrome (HUS), in which
the red blood cells are destroyed and the kidneys fail. About 2-7% of
infections lead to this complication. HUS is a combination of
microangiopathic hemolytic anemia, thrombocytopenia, and acute renal
failure. In the United States, HUS is the principal cause of acute kidney
failure in children, and most cases of HUS are caused by E. coli
O157:H7.
Escherichia coli is a gram negative bacterium which has hundreds
of serotypes which are mainly found in intestines of warm-blooded
vertebrates. Strains of E. coli are grouped by the pathogenic
mechanism of disease: enterohemorrhagic, enteroinvasive, enteropathogenic,
entertoxigenic, enteroaggregative, and enteroadherent
The most commonly recognized enterohemorrhagic E. coli (EHEC) is
E. coli O157:H7. Enterohemorrhagic strains produce a Shiga toxin
which damages endothelial cells. The bacteria are classified by their cell
wall (O antigen) and flagella antigen (H antigen).
The main reservoir for E. coli O157:H7 is the intestines of
healthy cattle. E. coli O157:H7 does not cause illness in cattle,
but there is still no way to get rid of the bacterium.
E. coli O157:H7 is excreted in feces of infected cattle, humans,
and other infected animals. It can be transmitted by a number of routes:
foodborne, waterborne, and person-to-person. Undercooked beef (i.e.
hamburgers), cross contamination or fecal contamination of food or water,
and consumption of raw milk are the most common sources of outbreaks.
The range is two to eight days, and the median is three to four days.
E. coli is shed in the stool during the initial period of
diarrhea and variably thereafter. Children can shed E. coli O157:H7
for two to four weeks after onset. Adults have a shorter infectious
period, and it has been reported that they can excrete E. coli for
up to three months.
Rapid investigation of single cases and clusters of E. coli
O157:H7 is critical to early recognition of larger outbreaks. Outbreak
recognition and investigation requires timely and complete epidemiological
investigation (risk factors, food history, history of exposure to animals,
etc.) paired with timely and complete laboratory investigation (serotyping
and PFGE). With the use of modern laboratory techniques, outbreaks may be
defined as three or more epi-linked cases infected with E. coli of
the same serotype and PFGE pattern.
Rapid institution of control measures in the early stages of outbreak
investigations is critical with this disease. In general, err on the side
of aggressive intervention.
Managing Possible Cases
- If the outbreak is linked to a public gathering or restaurant:
- Likely sources are undercooked meat, cross-contaminated food, or
possibly food contaminated by an infected food handler.
Environmental investigation should focus on specific food items and
method of preparation.
- Environmental inspection of the dairy or water supply is also
important.
- If an outbreak is linked to raw milk or milk products:
- Conduct an environmental evaluation of the dairy or water
facility.
- Impound any remaining products.
- If food was served at a public gathering:
- Identify any individuals who prepared food to see if they had any
diarrhea in the previous month.
- Identify any attendees who had diarrhea within two to eight days
after the gathering.
- Impound any remaining food.
- If an outbreak occurs at a day care or health care facility:
- Exclude all symptomatic individuals.
- In some situations, screening of asymptomatic attendees may also
be helpful.
- Investigate the possibility of person-to-person spread, foodborne
or waterborne spread, or direct contact with farm animals.
Clinical Description of E. coli
An illness that causes diarrhea (often bloody) and abdominal cramps.
The illness may be complicated by hemolytic uremic syndrome (HUS) or
thrombotic thrombocytopenic purpura (TTP).
Laboratory Criteria for Diagnosis of E. coli
- Isolation of E. coli O157:H7 from a clinical specimen.
- Isolation of Shiga toxin producing E. coli O157 from a
clinical specimen.
Case Classification of E. coli
Suspected: a case of post-diarrheal HUS or TTP
Probable:
- A case with isolation of E. coli O157:H7 from a clinical
specimen, pending confirmation of H7 or Shiga toxin.
- A clinically compatible case that is epidemiologically linked to a
confirmed or probable case.
- Identification of Shiga toxin in a specimen from a clinically
compatible case.
- Definitive evidence of an elevated antibody titer to a known EHEC
serotype from a clinically compatible case.
Confirmed: a case that meets the laboratory criteria for
diagnosis.
Clinical Description of Hemolytic Uremic Syndrome (HUS)
HUS is characterized by the acute onset of microangiopathic hemolytic
anemia, renal injury, and low platelet count. Thrombotic thrombocytopenic
purpura (TTP) is characterized by these features but can also involve the central nervous system (CNS). Fever may be present
in TTP, and there may be a more
gradual onset. Most cases of HUS (but a few cases of TTP) occur after an
acute gastrointestinal illness (usually diarrheal).
Laboratory Criteria for Diagnosis of HUS
The following are both present at some time during the illness:
- Anemia (acute onset) with microangiopathic changes (i.e.,
schistocytes, burr cells, or helmet cells) on peripheral blood
smear, and
- Renal injury with either hematuria, proteinuria, or elevated
creatinine level (>1.0 mg/dl in a child less than 13 years of age
and >1.5 mg/dl in anyone older than 13 years of age).
Case Classification of HUS
Probable:
- An acute illness diagnosed as HUS or TTP that meets the laboratory
criteria in a patient who does not have a clear history of acute or
bloody diarrhea in the preceding three weeks, or
- An acute illness diagnosed as HUS or TTP that
- Has onset within three weeks after onset of acute or bloody
diarrhea, and
- Meets the laboratory criteria except that microangiopathic
changes are not confirmed.
Confirmed: An acute illness diagnosed as HUS or TTP that meets
the laboratory criteria and began within three weeks after onset of an
episode of acute or bloody diarrhea.
The Office of Laboratory Services accepts stool specimens for EHEC.
Laboratory surveillance for EHEC is critical. Local health departments
should establish that laboratories in their jurisdiction screen all stools
for Shiga toxin producing E. coli by one of two methods. The
simplest method for most small labs is placing stool on SMAC followed by
latex agglutination testing for O157 antigen. Larger labs may consider EIA
screening of stools. If this method is chosen, please consult the Office
of Laboratory Services to arrange testing of the broth/slant for Shiga
toxin producing EHEC. Specimens should be submitted for
confirmation/identification and PFGE. Local health departments are
encouraged to routinely submit all EHEC isolates for testing to West
Virginia Office of Laboratory Services, 167 11th Avenue, South Charleston,
WV 25303.
Confirmation is based on laboratory findings, and clinical illness is
not required.
Share these prevention messages:
- Always wash hands with soap and water:
- after using the bathroom,
- after changing diapers,
- after cleaning the toilet,
- after handling soiled towels or linens,
- before eating, and
- after petting or handling animals.
- Drink only pasteurized milk products, fruit juices, and cider.
- Eat only fruits and vegetables that have been washed well.
- Follow these simple food preparation tips:
- Use a separate cutting board to prepare raw meats.
- Cook all ground beef and hamburger thoroughly. Ground beef should
be cooked to at least 160° F. If the temperature cannot be checked,
cook ground beef until the juices run clear, and the inside is gray
or brown throughout (not pink).
- Avoid spreading harmful bacteria in your kitchen. Use a clean
plate for cooked meat. Never return cooked meat back to the same
plate used for raw meat. Keep raw meat separate from ready-to-eat
foods. Wash hands, counters, and utensils with hot soapy water after
they touch raw meat. Never place cooked hamburgers or ground beef on
the unwashed plate that held raw patties. Wash meat thermometers in
between tests of patties that require further cooking.
- If you are served an undercooked hamburger in a restaurant, send
it back for further cooking.
- Marinade or BBQ sauce used on raw meat should not be used on
cooked meat.
- Persons who have diarrhea from any cause should not prepare food
that will be eaten by others, attend day care, or bathe or swim with
others.
No specific therapy will reduce the duration of illness. Most persons
recover without antibiotics or other specific treatment in five to 10
days. There is no evidence that antibiotics improve the course of disease,
and it is thought that treatment with some antibiotics may precipitate
kidney complications. Anti-diarrheal agents, such as loperamide (Imodium),
should also be avoided. Sulfa drugs such as TMP-SMX are contraindicated
because of the increased risk of developing HUS. When vomiting or diarrhea
are severe, rehydration may be indicated.
Hemolytic uremic syndrome is a life-threatening condition usually
treated in an intensive care unit. Blood transfusions and kidney dialysis
are often required. With intensive care, the death rate for hemolytic
uremic syndrome is 3-5%.
- Proportion of investigations with complete demographic information.
- Proportion of investigations with complete information on high-risk
occupations.
- Proportion of cases with laboratory confirmation and completed PFGE.
- Proportion of cases with complete risk factor investigation
including a two- to eight-day food history.
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