Office Of Labatory Services

MICROBIOLOGY


Our mission is to provide quality public health microbiological services for our providers and to support public health activities in control and prevention of communicable diseases.

Agencies served include County Health Departments, private physicians, hospitals including VA medical centers and private laboratories.


BACTERIOLOGY

  • Identifies bacteria referred from other agencies.
  • Serotypes Salmonella, Shigella, Neisseria meningitidis, Haemophilus influenzae, Streptococcus pneumoniae and Escherichia coli.
  • Provides bacteriological laboratory support for epidemiological investigation of suspected foodborne disease outbreaks.
  • Processes Jembec agar plate culture and urogenitial slides for Neisseria gonorrhea (GC).
  • Perform culture identification and PCR testing for Bordetella pertussis (whooping cough).
  • Examines autopsy materials from the WV Office of the Chief Medical Examiner.
  • Performs culturing of stool samples for the isolation and identification of enteric pathogens associated with foodborne diseases including Salmonella, Shigella, Campylobacter, Escherichia coli.
  • Refers unusual specimens to the Centers for Disease Control and Prevention (CDC) in Atlanta.

Bacteriology specimen collection instructions and required form(s)

Transport Guidelines for Fastidious Organisms


PARASITOLOGY

  • Processes stool specimens for detection and identification of intestinal parasites which includes, but are not limited to: protozoans, nematodes, cestodes and trematodes (roundworms, tapeworms and flukes).
  • Examines cellulose tape mounts for the presence or absence of pinworms (Enterobius vermicularis) and pinworm eggs.
  • Refers blood smears for malaria and other blood parasites to CDC.

Parasitology specimen collection instructions and required form(s)

FOODS

  • Performs examination of food products for bacterial contamination.

Foods specimen collection instructions and required form(s)

Prior approval is required for Food Testing. 
Please contact Microbiology at 304-558-3530 x2602


MOLECULAR

  • Performs Pulsed-Field Gel Electrophoresis (PFGE) on possible foodborne disease outbreak specimens.  Routine surveillance is performed on all Salmonella serotypes, Shigella species and Escherichia coli.  Other organisms are examined as needed.
  • Results are submitted to PulseNet (The National Molecular Subtyping Network for Foodborne Disease Surveillance) and Epidemiology.
  • Perform RT-PCR testing for Noroviruses

Collection and Transport Guidelines for Norovirus Testing

 

REFERRAL TESTING

(effective as of November 7, 2016)

 

While the Office of Laboratory Services makes every attempt to offer our clients the most complete menu of test methods, there are some tests that are not in high enough demand for it to be feasible for us to implement.  In these cases, we offer testing referral services.  The table below lists the most common referral tests requested.  For additional information about collection and shipping or to inquire about other tests, please contact OLS at 304-558-3530.

 

NOTE: Before shipping, submitters must contact the Division of Infectious Disease Epidemiology (DIDE) for consultation and approval.  Call 304-558-5358 and ask for the epidemiologist on call.

 

ANALYTE

METHOD

SPECIMEN TYPE

COLLECTION

AND STORAGE

CONDITIONS

TEST PERFORMED BY

Measles virus

PCR

Nasopharyngeal swab, throat swab, or urine (min. 250µL)

SWAB – collect and store in viral transport media at 4°C if shipping within 24 hours.  Ship on cold packs.

URINE – collect and store in sterile screw-top container at 4°C.  Do not add viral transport media.  Ship on cold packs.

Wisconsin State Laboratory of Hygiene

Measles virus

Serology

Serum (min. 1mL)

[NOTE: whole blood will not be accepted]

Collect blood in red-top or tiger-top tube and centrifuge.  Remove serum and transfer to sterile tube.  Store at 4°C and ship on cold packs.

CDC

Mumps virus

PCR

Buccal swab, nasopharyngeal swab, or throat swab

Collect and store in viral transport media at 4°C if shipping within 24 hours.  Ship on cold packs.

Wisconsin State Laboratory of Hygiene

Varicella-zoster virus

PCR

Skin lesion swab or scab

Dry swab only.  Store at ambient temperature.

Wisconsin State Laboratory of Hygiene

Chikungunya virus

PCR

Serum or CSF (min. 0.5mL)

[NOTE: whole blood will not be accepted]

Collect blood in red-top or tiger-top tube and centrifuge.  Remove serum and transfer to sterile tube.  Collect CSF in sterile tube.  Store both at 4°C and ship on cold packs.

CDC

Dengue virus

Serology and PCR

Serum (min. 1mL)

[NOTE: whole blood will not be accepted]

Collect blood in red-top or tiger-top tube and centrifuge.  Remove serum and transfer to sterile tube.  Store at 4°C and ship on cold packs.

CDC

Puerto Rico

Clostridium botulinum toxin

Serology and PCR

Foodborne: serum (min. 5mL), stool (min. 10g), vomitus, gastric contents, and food (INFANTS: stool and rectal swabs only)

 

Wound: serum, debrided tissue, and swab from wounds.

Collect blood in red-top or tiger-top tube and centrifuge.  Remove serum and transfer to sterile tube.  Collect and store stool in sterile screw-top container without preservative.  Collect tissue and wound swabs using dry swab only.  Store all specimens at 4°C and ship on cold packs.

CDC

Legionella spp. (Legionnaire’s Disease)

Culture, serogrouping, and PCR

Multiple specimen types -

(see

http://www.cdc.gov/legionella/downloads/shipping-instructions.pdf for complete instructions)

CDC


RELATED LINKS

WV Epidemiology Guidelines and Protocols (IDEP)
Health Topics - Provides information on topics significant to health and well-being.
Disease Listings - Provides an alphabetical list of diseases with both technical and general information.
FDA - Provides information on food safety.
CDC Division of Parasitic Diseases - Provides information on parasitic diseases.


CONTACT INFORMATION

Microbiology Section
Ph. 304-558-3530 x2602
Fx. 304-558-6210

Christi Clark M (ASCP)
Microbiologist Supervisor
christi.d.clark@wv.gov
304-558-3530 x2610


Page Last Updated: Nov 07, 2016
Site Last Updated: Oct 23, 2017


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